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Opt Express. 2009 Aug 31;17(18):15585-91. doi: 10.1364/OE.17.015585.

Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells.

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1
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University Durham, North Carolina 27708, USA. natan.shaked@duke.edu

Abstract

We present a phase-shifting interferometric technique for imaging live biological cells in growth media, while optimizing spatial resolution and enabling potential real-time measurement capabilities. The technique uses slightly-off-axis interferometry which requires less detector bandwidth than traditional off-axis interferometry and fewer measurements than traditional on-axis interferometry. Experimental and theoretical comparisons between the proposed method and these traditional interferometric approaches are given. The method is experimentally demonstrated via phase microscopy of live human skin cancer cells.

PMID:
19724557
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