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Pediatr Dent. 2009 Jul-Aug;31(4):334-9.

Effect of salivary contamination on microleakage of resin composites placed with a self-etch adhesive in primary teeth: an in vitro study.

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Department of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.



The purpose of this study was to investigate in vitro the effect of salivary contamination on the microleakage of resin composite restorations placed with a self-etching adhesive in primary teeth.


Fifty-six extracted primary molars received standardized Class V preparations. The teeth were assigned to 7 groups (n=8): 1-layer and 2-layer application of the self-etching primer (Clearfil SE Bond) with no contamination (groups 1 and 2, respectively); saliva, SE primer, and drying (group 3); SE primer, drying, saliva, and drying (group 4); SE primer, drying, saliva, and rinsing/drying (group 5); SE primer, drying, saliva, drying, SE primer, and drying (group 6); and SE primer, drying, saliva, rinsing/drying, SE primer, and drying (group 7). For all specimens, the bonding agent (SE adhesive) and a composite were applied. After thermocycling and immersing in basic fuchsin, the teeth were sectioned and evaluated under a stereomicroscope. Data were analyzed using Kruskal-Wallis and Mann-Whitney tests.


The mean occlusal and gingival microleakage for groups 4 and 5 were significantly higher than that of groups 1 and 2 (P<.04). No statistically significant difference in microleakage among groups 1, 2, 6, and 7 was found (P=.09). The degree of microleakage in the gingival margins was significantly higher than that of occlusal margins (P=.027).


The self-etching adhesive was not sensitive to saliva when contamination occurred before primer application.

[Indexed for MEDLINE]

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