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Bioresour Technol. 2010 Jan;101(1):285-91. doi: 10.1016/j.biortech.2009.07.057. Epub 2009 Aug 31.

Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant.

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1
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China. yuhm@tsinghua.edu.cn

Abstract

Microbial transformation of acrylonitrile to acrylamide by nitrile hydratase is of great interest to green chemistry. During the transformation, acrylic acid is generally accumulated as a by-product through biocatalysis of amidase. A novel strain with high nitrile hydratase activity was isolated from soil and identified as Rhodococcus ruber TH by morphology and 16S rRNA gene analysis. An amidase-negative recombinant, R. ruber TH3 was constructed. Its nitrile hydratase activity was 25% higher than that of the wild type, reaching 490+/-29.2U/mg dry cell weight, while the amidase activity was 60% lower. After 6 h hydration of acrylonitrile using free cells as biocatalysts at 18 degrees C, acrylamide production by TH3 was 23% higher and the production of the by-product, acrylic acid, was 87% lower than that of the wild type. This result demonstrates that the strain TH3 could be valuable for industrial applications.

PMID:
19720524
DOI:
10.1016/j.biortech.2009.07.057
[Indexed for MEDLINE]
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