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FEMS Microbiol Ecol. 2003 Feb 1;43(1):55-61. doi: 10.1111/j.1574-6941.2003.tb01045.x.

Flow cytometry discrimination between bacteria and clay-humic acid particles during growth-linked biodegradation of phenanthrene by Pseudomonas aeruginosa 19SJ.

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Departament de Microbiologia, Universitat de Barcelona, Avenida Diagonal 645, 08028 Barcelona, Spain.

Abstract

Methods that quickly assess microbial density and aggregation in soil and sediments are needed in environmental microbiology. We report a flow cytometry method that uses the green and orange emission of the fluorochrome SYTO-13 to discriminate between bacteria and clay-humic acid particles. This approach distinguishes single or clustered bacteria, and clusters of bacteria and abiotic particles during the growth of the biosurfactant-producing strain Pseudomonas aeruginosa 19SJ on solid phenanthrene in the presence of humic acid-clay complexes.

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