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PLoS One. 2009 Aug 31;4(8):e6869. doi: 10.1371/journal.pone.0006869.

Glioblastoma formation from cell population depleted of Prominin1-expressing cells.

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Department of Biology, Graduate School of Science, Kobe University, Kobe, 657-8601, Japan. [corrected]

Erratum in

  • PLoS One. 2009;4(9). doi: 10.1371/annotation/d1e654f7-c02d-458c-8dc7-da7ae96aa594.


Prominin1 (Prom1, also known as CD133 in human) has been widely used as a marker for cancer stem cells (CSCs), which self-renew and are tumorigenic, in malignant tumors including glioblastoma multiforme (GBM). However, there is other evidence showing that Prom1-negative cancer cells also form tumors in vivo. Thus it remains controversial whether Prom1 is a bona fide marker for CSCs. To verify if Prom1-expressing cells are essential for tumorigenesis, we established a mouse line, whose Prom1-expressing cells can be eliminated conditionally by a Cre-inducible DTA gene on the Prom1 locus together with a tamoxifen-inducible CreER(TM), and generated glioma-initiating cells (GICs-LD) by overexpressing both the SV40 Large T antigen and an oncogenic H-Ras(L61) in neural stem cells of the mouse line. We show here that the tamoxifen-treated GICs-LD (GICs-DTA) form tumor-spheres in culture and transplantable GBM in vivo. Thus, our studies demonstrate that Prom1-expressing cells are dispensable for gliomagenesis in this mouse model.

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