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Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368. Epub 2009 Aug 30.

Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis.

Author information

1
Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.

Abstract

We developed genetically encoded fluorescence resonance energy transfer (FRET)-based sensors that display a large ratiometric change upon Zn(2+) binding, have affinities that span the pico- to nanomolar range and can readily be targeted to subcellular organelles. Using this sensor toolbox we found that cytosolic Zn(2+) was buffered at 0.4 nM in pancreatic beta cells, and we found substantially higher Zn(2+) concentrations in insulin-containing secretory vesicles.

PMID:
19718032
PMCID:
PMC6101214
DOI:
10.1038/nmeth.1368
[Indexed for MEDLINE]
Free PMC Article

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