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Nat Methods. 2009 Oct;6(10):733-5. doi: 10.1038/nmeth.1369. Epub 2009 Aug 30.

Somatic cell nuclear transfer in zebrafish.

Author information

1
Comparative Medicine and Integrative Biology, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA.

Abstract

We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.

PMID:
19718031
DOI:
10.1038/nmeth.1369
[Indexed for MEDLINE]

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