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Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Sep;108(3):458-64. doi: 10.1016/j.tripleo.2009.05.006.

Cytotoxic effect of a 35% hydrogen peroxide bleaching gel on odontoblast-like MDPC-23 cells.

Author information

1
Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, São Paulo State University, Araraquara, Brazil.

Abstract

OBJECTIVE:

This study evaluated transenamel and transdentinal cytotoxic effects of a bleaching gel on the MDPC-23 cell line.

STUDY DESIGN:

Discs obtained from bovine incisors were placed in a metallic device to simulate an in vivo pulp chamber. Groups were formed according to the enamel surface treatment: G1: 35% H(2)O(2) bleaching gel; G2: 35% H(2)O(2) bleaching gel + halogen light; G3: halogen light; and G4: control. Cell metabolism was evaluated by the methyltetrazolium assay and cell morphology by scanning electron microscopy.

RESULTS:

Cell metabolism decreased by 31.7%, 41.6%, and 11.5% in G1, G2, and G3, respectively. Cytotoxic effects observed in G2 were significantly more severe compared with G3 and G4. In G1 and G2, a smaller number of viable cells with major morphologic alterations remained adhered to dentin.

CONCLUSION:

The bleaching gel associated with light presented transenamel and transdentinal cytotoxic effects characterised by direct damage to odontoblasts and decrease of their metabolic activity.

PMID:
19716511
DOI:
10.1016/j.tripleo.2009.05.006
[Indexed for MEDLINE]

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