Format

Send to

Choose Destination
Chem Biol. 2009 Aug 28;16(8):868-81. doi: 10.1016/j.chembiol.2009.07.009.

Noninflammatory gluten peptide analogs as biomarkers for celiac sprue.

Author information

1
Department of Biochemistry, Stanford University, CA 94305, USA.

Abstract

New tools are needed for managing celiac sprue, a lifelong immune disease of the small intestine. Ongoing drug trials are also prompting a search for noninvasive biomarkers of gluten-induced intestinal change. We have synthesized and characterized noninflammatory gluten peptide analogs in which key Gln residues are replaced by Asn or His. Like their proinflammatory counterparts, these biomarkers are resistant to gastrointestinal proteases, susceptible to glutenases, and permeable across enterocyte barriers. Unlike gluten peptides, however, they are not appreciably recognized by transglutaminase, HLA-DQ2, or disease-specific T cells. In vitro and animal studies show that the biomarkers can detect intestinal permeability changes as well as glutenase-catalyzed gastric detoxification of gluten. Accordingly, controlled clinical studies are warranted to evaluate the use of these peptides as probes for abnormal intestinal permeability in celiac patients and for glutenase efficacy in clinical trials and practice.

PMID:
19716477
PMCID:
PMC3721637
DOI:
10.1016/j.chembiol.2009.07.009
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central Icon for Norwegian BIBSYS system
Loading ...
Support Center