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J Nutr Biochem. 2010 Aug;21(8):695-701. doi: 10.1016/j.jnutbio.2009.04.003. Epub 2009 Aug 27.

Beta-glucan extracts inhibit the in vitro intestinal uptake of long-chain fatty acids and cholesterol and down-regulate genes involved in lipogenesis and lipid transport in rats.

Author information

1
Nutrition and Metabolism Group, University of Alberta, Edmonton, Alberta, Canada.

Abstract

BACKGROUND:

Dietary fiber reduces the intestinal absorption of nutrients and the blood concentrations of cholesterol and triglycerides.

AIM:

We wished to test the hypothesis that high-viscosity (HV) and low-viscosity preparations of barley and oat beta-glucan modify the expression of selected genes of lipid-binding proteins in the intestinal mucosa and reduce the intestinal in vitro uptake of lipids.

METHODS:

Five different beta-glucan extracts were separately added to test solutions at concentrations of 0.1-0.5% (wt/wt), and the in vitro intestinal uptake of lipids into the intestine of rats was assessed. An intestinal cell line was used to determine the effect of beta-glucan extracts on the expression of intestinal genes involved in lipid metabolism and fatty acid transport.

RESULTS:

All extracts reduced the uptake of 18:2 when the effective resistance of the unstirred water layer was high. When the unstirred layer resistance was low, the HV oat beta-glucan extract reduced jejunal 18:2 uptake, while most extracts reduced ileal 18:2 uptake. Ileal 18:0 uptake was reduced by the HV barley extract, while both jejunal and ileal cholesterol uptakes were reduced by the medium-purity HV barley extract. The inhibitory effect of HV barley beta-glucan on 18:0 and 18:2 uptake was more pronounced at higher fatty acid concentrations. The expression of genes involved in fatty acid synthesis and cholesterol metabolism was down-regulated with the HV beta-glucan extracts. beta-Glucan extracts also reduced intestinal fatty-acid-binding protein and fatty acid transport protein 4 mRNA.

CONCLUSIONS:

The reduced intestinal fatty acid uptake observed with beta-glucan is associated with inhibition of genes regulating intestinal uptake and synthesis of lipids. The inhibitory effect of beta-glucan on intestinal lipid uptake raises the possibility of their selective use to reduce their intestinal absorption.

PMID:
19716281
PMCID:
PMC3833848
DOI:
10.1016/j.jnutbio.2009.04.003
[Indexed for MEDLINE]
Free PMC Article

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