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Vaccine. 2009 Oct 19;27(44):6137-42. doi: 10.1016/j.vaccine.2009.08.028. Epub 2009 Aug 26.

HIV inactivation by cross-linking of photo-labeled anti-retroviral compounds with HIV reverse transcriptase.

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McGill AIDS Center, Lady Davis Institute, Jewish General Hospital, 3755 Côte Ste. Catherine, Montréal, Canada H3T-1E2.


We describe a new method for the development of a preventive inactivated-HIV vaccine, based on photo-inactivation of HIV reverse transcriptase (RT), which preserves both the conformational and functional integrity of viral surface proteins. The RT of HIV-1 was selectively targeted for inactivation using a photo-labeled compound with specific affinity for HIV-1 RT. The photo-labeled virions were then exposed to UV light causing the photo-labeled compound to form a covalent bond cross-linking the photo-active compound to RT. Replication capacity of the treated virions was significantly reduced when compared to controls suggesting that exposure of treated virions to UV light had caused a stable interaction of RT and the photo-labeling compound.

[Indexed for MEDLINE]

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