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Vaccine. 2009 Nov 5;27(47):6605-12. doi: 10.1016/j.vaccine.2009.08.016. Epub 2009 Aug 25.

Induction of HIV-1 subtype B and AE-specific neutralizing antibodies in mice and macaques with DNA prime and recombinant gp140 protein boost regimens.

Author information

1
Department of Microbiology and Immunology, University of Melbourne, Parkville, 3010 VIC, Australia.

Abstract

We developed highly expressing clade B and AE DNA and envelope protein (Env) vaccines for evaluation in mice and macaques as DNA prime/protein boost regimens. High levels of Env-specific antibodies were induced in mice, albeit with limited neutralizing activity in vitro. A combined clade B and AE regimen induced high titer Env-specific antibody in two pigtail macaques that neutralized several strains of HIV-1. However, upon mucosal challenge with SHIV(SF162P3) no protection from infection was observed. Although the vaccines tested provide a platform for inducing robust humoral immunity, further refinements to broaden coverage against divergent strains and induce mucosal immunity are needed.

PMID:
19712773
DOI:
10.1016/j.vaccine.2009.08.016
[Indexed for MEDLINE]

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