Susceptibility of 50 isolates of Bacillus anthracis to 24 antibiotics was tested by the disk-diffusion method and the method of serial dilutions in solid media. The tests allowed to determine the boundary values of the growth inhibition zones and the minimum inhibitory concentrations of the antibiotics for susceptible and resistant strains of B. anthracis. Nutrient media and reference strains for testing antibiotic susceptibility of B. anthracis are recommended.