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J Neurosci Methods. 1990 Apr;32(1):63-70.

A chemically-defined medium for organotypic slice cultures.

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1
Department of Anatomy and Neurobiology, College of Medicine, University of California, Irvine 92717.

Abstract

Organotypic slice cultures provide an excellent system for the analysis of study of the molecular mechanisms of this development necessitates the use of a chemically defined culture medium. We report here the development of a medium, EOL1 defined medium, designed specifically for this purpose. Cultures of both cerebral cortex and basal forebrain demonstrate that this defined medium allows a high degree of cytoarchitectural maintenance while promoting neural metabolism and process outgrowth.

PMID:
1970842
[Indexed for MEDLINE]

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