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Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14367-72. doi: 10.1073/pnas.0901074106. Epub 2009 Aug 13.

Structural waters define a functional channel mediating activation of the GPCR, rhodopsin.

Author information

1
Department of Pharmacology, Center for Proteomics and Bioinformatics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106-4965, USA.

Abstract

Structural water molecules may act as prosthetic groups indispensable for proper protein function. In the case of allosteric activation of G protein-coupled receptors (GPCRs), water likely imparts structural plasticity required for agonist-induced signal transmission. Inspection of structures of GPCR superfamily members reveals the presence of conserved embedded water molecules likely important to GPCR function. Coupling radiolytic hydroxyl radical labeling with rapid H(2)O(18) solvent mixing, we observed no exchange of these structural waters with bulk solvent in either ground state or for the Meta II or opsin states. However, the radiolysis approach permitted labeling of selected side chain residues within the transmembrane helices and revealed activation-induced changes in local structural constraints likely mediated by dynamics of both water and protein. These results suggest both a possible general mechanism for water-dependent communication in family A GPCRs based on structural conservation, and a strategy for probing membrane protein structure.

PMID:
19706523
PMCID:
PMC2732891
DOI:
10.1073/pnas.0901074106
[Indexed for MEDLINE]
Free PMC Article

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