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J Agric Food Chem. 2009 Sep 23;57(18):8379-85. doi: 10.1021/jf901618z.

DNA barcoding of commercially important salmon and trout species (Oncorhynchus and Salmo) from North America.

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  • 1Oregon State University Seafood Lab, Department of Food Science and Technology, Oregon State University, Astoria, Oregon 97103, USA. rosalee.rasmussen@oregonstate.edu

Abstract

The present study investigated the ability of DNA barcoding to reliably identify the seven commercially important salmon and trout species (genera Oncorhynchus and Salmo ) in North America. More than 1000 salmonid reference samples were collected from a wide geographic range. DNA extracts from these samples were sequenced for the standard 650 bp barcode region of the cytochrome c oxidase subunit I gene (COI). DNA barcodes showed low intraspecies divergences (mean, 0.26%; range, 0.04-1.09%), and the mean congeneric divergence was 32-fold greater, at 8.22% (range, 3.42-12.67%). The minimum interspecies divergence was always greater than the maximum intraspecies divergence, indicating that these species can be reliably differentiated using DNA barcodes. Furthermore, several shorter barcode regions (109-218 bp), termed "mini-barcodes", were identified in silico that can differentiate all eight species, providing a potential means for species identification in heavily processed products.

PMID:
19705801
DOI:
10.1021/jf901618z
[PubMed - indexed for MEDLINE]
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