Format

Send to

Choose Destination
Plant Signal Behav. 2007 Sep;2(5):416-20.

Arabidopsis protein microarrays for the high-throughput identification of protein-protein interactions.

Author information

1
Department of Molecular, Cellular and Developmental Biology; Yale University; New Haven, Connecticut USA.

Abstract

Protein microarray technology has emerged as a powerful new approach for the study of thousands of proteins simultaneously. Protein microarrays have been used for a wide variety of applications for the human and yeast systems. In a recent study, we demonstrated that Arabidopsis functional protein microarrays can be generated and employed to characterize the function of plant proteins. The arrayed proteins were produced using an optimized large-scale plant-based expression system. In a proof-of concept study, 173 known and novel potential substrates of calmodulin (CaM) and calmodulin-like proteins (CML) were identified in an unbiased and high-throughput manner. The information documented here on novel potential CaM targets provides new testable hypotheses in the area of CaM/Ca(2+)-regulated processes and represents a resource of functional information for the scientific community.

KEYWORDS:

calmodulin; large scale protein production; protein microarray; protein-protein interactions

PMID:
19704619
PMCID:
PMC2634232

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center