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Vaccine. 2009 Dec 10;28(1):279-89. doi: 10.1016/j.vaccine.2009.08.017. Epub 2009 Aug 22.

Epitope mapping immunodominant regions of the PilA protein of nontypeable Haemophilus influenzae (NTHI) to facilitate the design of two novel chimeric vaccine candidates.

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The Research Institute at Nationwide Children's Hospital, Center for Microbial Pathogenesis and The Ohio State University College of Medicine, 700 Children's Drive, W591, Columbus, OH 43205, United States.


We designed and tested three PilA-derived vaccine candidates in a chinchilla model of ascending nontypeable Haemophilus influenzae (NTHI)-induced otitis media (OM). Delivery of antiserum directed against each immunogen conferred varying degrees of protection. Presentation of a B-cell epitope derived from the OMP P5 adhesin at the N-terminus of recombinant soluble PilA protein (as opposed to the C-terminus), resulted in a protective chimeric immunogen that combined epitopes from two distinct NTHI adhesins (type IV pili and OMP P5). Incorporating protective epitopes derived from two NTHI adhesins/virulence determinants into a single pediatric vaccine candidate to prevent OM has multiple potential inherent advantages.

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