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Toxicol In Vitro. 2010 Feb;24(1):135-41. doi: 10.1016/j.tiv.2009.08.004. Epub 2009 Aug 20.

Oxidative stress and apoptosis are induced in human endothelial cells exposed to urban particulate matter.

Author information

1
Department of Cell Biology, Instituto Nacional de Cardiología Ignacio Chávez, México D.F., Mexico.

Abstract

Correlations between exposure to particle matter (PM) with an aerodynamic diameter <or= 2.5 or 10microm (PM(2.5) and PM(10), respectively) with cardiovascular effects have been demonstrated recently. Endothelial cells seem to play a relevant role in the responses to PM due to their participation in pro-inflammatory events. In this study we determined the effect of PM(2.5) and PM(10) from Mexico City on human endothelial cells by means of evaluating reactive oxygen species (ROS), nitric oxide (NO), NF-kappaB translocation and cell death. For this purpose we used human umbilical vein endothelial cells (HUVEC) as a model. The production of ROS was determined by the reduction of H(2)DCFDA and NO by Griess reagent. The translocation of NF-kappaB was evaluated by Electrophoretic Mobility Shift Assay (EMSA) and the cellular death by the translocation of phosphatidylserine. TNF-alpha was used as a positive control for endothelial cell activation. PM(2.5) and PM(10) induced the production of ROS (77% and 126% increase, respectively, vs. control) and NO (up to 132% and 233% increase, respectively, vs. control). PM(2.5) and PM(10) also induced the nuclear translocation of NF-kappaB. All these events were associated with apoptosis. In conclusion, the activation of HUVEC induced by PM(2.5) and PM(10) is related with an oxidative stress, suggesting that these particles may participate in the development of cardiovascular and inflammatory diseases.

PMID:
19699290
DOI:
10.1016/j.tiv.2009.08.004
[Indexed for MEDLINE]

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