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Chest. 2009 Nov;136(5):1291-1300. doi: 10.1378/chest.08-2767. Epub 2009 Aug 20.

Systemic and pulmonary oxidative stress after single-leg exercise in COPD.

Author information

1
Department of Respiratory Medicine, Maastricht University, Maastricht, the Netherlands. Electronic address: e.mercken@pul.unimaas.nl.
2
Department of Respiratory Medicine, Maastricht University, Maastricht, the Netherlands.
3
Centre for Integrated Rehabilitation Organ Failure, Horn, the Netherlands.
4
Centre for Integrated Rehabilitation Organ Failure, Horn, the Netherlands; Department of Respiratory Medicine, Maastricht University, Maastricht, the Netherlands.
5
Department of Pharmacology and Toxicology, Maastricht University, Maastricht, the Netherlands.
6
Department of Health Risk Analysis and Toxicology, Maastricht University, Maastricht, the Netherlands.

Abstract

BACKGROUND:

Our aim for this study was to disentangle the contribution of muscular vs pulmonary oxidative stress during endurance exercise in patients with COPD.

METHODS:

Fifteen COPD patients and 10 healthy age-matched control subjects performed a continuously submaximal single-leg ergometer test (40% of peak workload) for 20 min or until they stopped (muscle endurance [Tlim]). Venous blood, urine samples, and exhaled breath condensate were sampled before, immediately after, and 2 h after exercise.

RESULTS:

Tlim was lower in COPD patients than in control subjects (p < 0.01). No exercise-induced systemic inflammation (ie, no raised levels of interleukin-6 or tumor necrosis factor-alpha) was found in the groups. Urinary malondialdehyde and uric acid levels (p < 0.05) were increased in COPD patients, whereas erythrocyte oxidized glutathione/reduced glutathione levels tended to be increased in COPD patients compared with control subjects after exercise (p = 0.08). Despite the relatively low cardioventilatory response to this localized muscle exercise, hydrogen peroxide levels in breath condensate significantly increased in COPD patients (p < 0.01). Nuclear factor kappaB DNA-binding activity of p50 in peripheral blood monocytes was elevated after exercise in both COPD patients (p < 0.01) and control subjects (p < 0.05), whereas p65 protein levels were not altered.

CONCLUSION:

COPD patients showed increased pulmonary and systemic oxidative stress after localized leg muscle exercise compared with healthy control subjects, without evidence of increased levels of systemic inflammation.

PMID:
19696125
DOI:
10.1378/chest.08-2767
[Indexed for MEDLINE]

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