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Cell Stress Chaperones. 2010 Mar;15(2):219-24. doi: 10.1007/s12192-009-0136-7. Epub 2009 Aug 20.

Protective effect of carbon monoxide pre-conditioning on LPS-induced endothelial cell stress.

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1
Department of Veterinary Morphophysiology and Animal Production, DIMORFIPA, University of Bologna, Italy. chiara.bernardini5@unibo.it

Abstract

Increasing evidence indicates that carbon monoxide (CO) may protect against several diseases including sepsis. The ability of CO pre-treatment to provide good pre-conditioning against lipopolysaccharide (LPS)-induced injury was tested using an in vitro model of primary culture of porcine aortic endothelial cells (pAEC). pAEC were exposed to CO (250 ppm) or air for 1 h prior to the addition of LPS (10 microg/ml). Hsp70, HO-1, and Egr-1 protein levels were determined as well as vascular endothelial growth factor (VEGF) secretion after 4, 7, and 15 h. The effect of CO on LPS-induced apoptosis was also detected at 15 h. CO pre-treatment before the addition of LPS, significantly reduced LPS-induced apoptosis. LPS induced an increase in the level of VEGF in culture media after 7 and 15 h, and a larger increase was detected in CO pre-treated cells. In addition, CO pre-treatment reduced LPS-induced Hsp70, HO-1, and Egr-1 protein expression. In conclusion, CO treatment seems to provide a good pre-conditioning for the prevention of LPS-induced endothelial injury.

PMID:
19693705
PMCID:
PMC2866981
DOI:
10.1007/s12192-009-0136-7
[Indexed for MEDLINE]
Free PMC Article
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