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J Virol. 2009 Nov;83(21):11402-6. doi: 10.1128/JVI.00504-09. Epub 2009 Aug 19.

Characterization of a mumps virus nucleocapsidlike particle.

Author information

1
Department of Microbiology, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, Alabama 35294, USA.

Abstract

The nucleocapsid protein (NP) of mumps virus (MuV), a paramyxovirus, was coexpressed with the phosphoprotein (P) in Escherichia coli. The NP and P proteins form a soluble complex containing RNA. Under a transmission electron microscope, the NP-RNA complex appears as a nucleocapsidlike ring that has a diameter of approximately 20 nm with 13 subunits. There is a piece of single-stranded RNA with a length of 78 nucleotides in the NP-RNA ring. Shorter RNA pieces are also visible. The MuV NP protein may provide weaker protection of the RNA than the NP protein of some other negative-strand RNA viruses, reflecting the degree of NP protein association.

PMID:
19692473
PMCID:
PMC2772791
DOI:
10.1128/JVI.00504-09
[Indexed for MEDLINE]
Free PMC Article

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