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Bioresour Technol. 2009 Dec;100(24):6143-8. doi: 10.1016/j.biortech.2009.07.035. Epub 2009 Aug 18.

Modulation of guanosine 5'-diphosphate-D-mannose metabolism in recombinant Escherichia coli for production of guanosine 5'-diphosphate-L-fucose.

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Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.


Guanosine 5'-diphosphate (GDP)-L-fucose, an activated form of a nucleotide sugar, plays an important role in a wide range of biological functions. In this study, the enhancement of GDP-L-fucose production was attempted by supplementation of mannose, which is a potentially better carbon source to be converted into GDP-L-fucose than glucose, and combinatorial overexpression of the genes involved in the biosynthesis of GDP-D-mannose, a precursor of GDP-L-fucose. Supply of a mannose and glucose led to a 1.3-fold-increase in GDP-L-fucose concentration (52.5+/-0.8 mg l(-1)) in a fed-batch fermentation of recombinant E. coli BL21star(DE3) overexpressing the gmd and wcaG genes, compared with the case using glucose as a sole carbon source. A maximum GDP-L-fucose concentration of 170.3+/-2.3 mg l(-1), corresponding to a 4.4-fold enhancement compared with the control strain overexpressing gmd and wcaG genes only, was achieved in a glucose-limited fed-batch fermentation of a recombinant E. coli BL21star(DE3) strain overexpressing manB, manC, gmd and wcaG genes. Further improvement of GDP-L-fucose production was not obtained by additional overexpression of the manA gene.

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