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Mol Biol Rep. 2010 Jun;37(5):2223-6. doi: 10.1007/s11033-009-9708-y. Epub 2009 Aug 19.

Use of zinc chloride as alternative stimulant for in vitro study of nitric oxide production pathway in avian splenocyte culture.

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1
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700037, India.

Abstract

Nitric Oxide (NO) plays an important role for regulation of cellular and vascular response of inflammation and initiates killing mechanism in the host-defense reactions. NO production is regulated through inducible nitric oxide synthase (iNOS) pathway in response to infections and injurious agents besides pro-inflammatory cytokines secreted by the host. Evaluation of NO pathway is one of the major targets which can evaluate various immunomodulators for their therapeutic interaction on innate immune system. Lipopolysaccharide (LPS) and concavalin A (ConA) are used as standard mitogen for peripheral blood mononuclear cells and splenocyte of mammalian and avian cell culture. During the present investigation ZnCl(2) has been explored as standard mitogen and result was comparable with standard mitogen. The result has been evaluated on the basis of relative mRNA expression of iNOS and interferon gamma and nitrite assay. Observation indicated significantly higher expression of both biomolecules in comparison to control, LPS, ConA treated group. This study indicated that, ZnCl(2) can also be used as standard stimulant for molecular mining of innate immunity.

PMID:
19690983
DOI:
10.1007/s11033-009-9708-y
[Indexed for MEDLINE]

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