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Methods Mol Biol. 2009;558:251-60. doi: 10.1007/978-1-60761-103-5_15.

Immunofluorescent microscopic study of meiosis in zebrafish.

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1
Department of Biology, York University, Toronto, Ontario, Canada.

Abstract

In recent years, zebrafish (Danio rerio) has been used as a model vertebrate organism for studies of human disease, development, and genetics. This chapter describes detailed methods for the preparation of whole-mount meiotic oocytes and spermatocytes as well as cryostat sectioning of ovaries and testes for immunofluorescence microscopy studies of zebrafish.

PMID:
19685329
DOI:
10.1007/978-1-60761-103-5_15
[Indexed for MEDLINE]
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