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Nat Methods. 2009 Sep;6(9):651-3. doi: 10.1038/nmeth.1365. Epub 2009 Aug 16.

Crystallographic ab initio protein structure solution below atomic resolution.

Author information

1
Instituto de Biología Molecular de Barcelona, Barcelona Science Park, Barcelona, Spain.

Abstract

Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 A) unless heavy atoms are present. We describe a general ab initio phasing method for 2 A data, based on combination of localizing model fragments such as small á-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 A.

PMID:
19684596
DOI:
10.1038/nmeth.1365
[Indexed for MEDLINE]

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