Format

Send to

Choose Destination
Nat Chem Biol. 2009 Oct;5(10):718-20. doi: 10.1038/nchembio.201. Epub 2009 Aug 16.

DNA-catalyzed sequence-specific hydrolysis of DNA.

Author information

1
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Abstract

Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn2+ and Zn2+, which is noteworthy because many natural DNA nucleases are bimetallic protein enzymes.

Comment in

PMID:
19684594
PMCID:
PMC2746877
DOI:
10.1038/nchembio.201
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center