Format

Send to

Choose Destination
Mol Cell. 2009 Aug 14;35(3):365-76. doi: 10.1016/j.molcel.2009.06.024.

yFACT induces global accessibility of nucleosomal DNA without H2A-H2B displacement.

Author information

1
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84112, USA.

Abstract

FACT has been proposed to function by displacing H2A-H2B dimers from nucleosomes to form hexasomes. Results described here with yeast FACT (yFACT) suggest instead that nucleosomes are reorganized to a form with the original composition but a looser, more dynamic structure. First, yFACT enhances hydroxyl radical accessibility and endonuclease digestion in vitro at sites throughout the nucleosome, not just in regions contacted by H2A-H2B. Accessibility increases dramatically, but the DNA remains partially protected. Second, increased nuclease sensitivity can occur without displacement of dimers from the nucleosome. Third, yFACT is required for eviction of nucleosomes from the GAL1-10 promoter during transcriptional activation in vivo, but the preferential reduction in dimer occupancy expected for hexasome formation is not observed. We propose that yFACT promotes a reversible transition between two nucleosomal forms, and that this activity contributes to the establishment and maintenance of the chromatin barrier as well as to overcoming it.

PMID:
19683499
PMCID:
PMC2748400
DOI:
10.1016/j.molcel.2009.06.024
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center