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J Immunol Methods. 2009 Oct 31;350(1-2):118-24. doi: 10.1016/j.jim.2009.08.002. Epub 2009 Aug 12.

DNA sensor development based on multi-wall carbon nanotubes for label-free influenza virus (type A) detection.

Author information

1
Hanoi Advanced School of Science and Technology, Hanoi University of Technology, Vietnam. tampd-hast@mail.hut.edu.vn

Abstract

This paper describes the DNA immobilization using carbon multi-walled nanotubes (MWCNTs) for direct and label-free detection of influenza virus (type A). The DNA probe was attached on the sensor surface by means of covalent bonding between the amine and phosphate groups of the DNA sequence. The interaction between the DNA probe and the MWCNTs were characterized by Fourier Transform Infrared (FTIR) spectrometry, Raman spectra. The hybridization of the DNA probe and the target DNA were detected by changes in the conductance on the surface of sensors leading to the change in the output signal of the system. The results show that the DNA sensor can detect as low as 0.5 nM of the target DNA samples; the response time of DNA sensor is approximately 4 min.

PMID:
19682995
DOI:
10.1016/j.jim.2009.08.002
[Indexed for MEDLINE]

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