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Diagn Microbiol Infect Dis. 2009 Sep;65(1):69-72. doi: 10.1016/j.diagmicrobio.2009.05.005.

Real-time polymerase chain reaction detection of Cryptococcus neoformans and Cryptococcus gattii in human samples.

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Laboratoire Hospitalo-universitaire de Parasitologie et Mycologie Médicale, Equipe EA3593, UFR de Médecine, Université des Antilles et de la Guyane, Cayenne, French Guiana 97 300.


We report a TaqMan real-time polymerase chain reaction (PCR) protocol for diagnosing cryptococcosis. Specificity was tested with 33 fungal strains. The 7 clinical samples found positive by culture also tested positive by real-time PCR. No false negatives were found among the 94 clinical samples that were negative by culture for Cryptococcus spp. The sensitivity threshold was about 10 plasmid copies per assay.

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