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Nat Chem Biol. 2009 Oct;5(10):715-7. doi: 10.1038/nchembio.200. Epub 2009 Aug 9.

Cell-selective metabolic labeling of proteins.

Author information

1
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, USA.

Abstract

Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.

PMID:
19668194
PMCID:
PMC3176724
DOI:
10.1038/nchembio.200
[Indexed for MEDLINE]
Free PMC Article

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