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Anal Sci. 2009 Aug;25(8):985-8.

Isomeric oligosaccharides analyses using negative-ion electrospray ionization ion mobility spectrometry combined with collision-induced dissociation MS/MS.

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1
SUNTORY Institute for Bioorganic Research, Mishima, Osaka, Japan. yumagaki@sunbor.or.jp

Abstract

Ion mobility-mass spectrometry (IM-MS) was applied to analyses of isomeric oligosaccharides linked by alpha1-4, alpha1-6, beta1-3, and beta1-4 glycosyl linkages. Negative-ion electrospray ionization (ESI) quadrupole IM time-of-flight mass spectrometry allowed the combination of IM separation and collision-induced dissociation (CID) MS/MS product ion analysis. Multimer formations of hexa-saccharide linkage isomers differ from each other, and their molecular shapes were analyzed by ion mobility spectrometry (IMS). The product ion spectra of the oligosaccharide isomers were measured by negative-ion CID-MS/MS for each IM separated peak. The spectrum for each isomer was distinct, and their corresponding linkage structures were identified by MS/MS analysis.

PMID:
19667474
[Indexed for MEDLINE]
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