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Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12794-7. doi: 10.1073/pnas.0905845106. Epub 2009 Jul 30.

A DNA barcode for land plants.

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1
Royal Botanic Garden Edinburgh, Edinburgh EH3 5LR, United Kingdom.

Abstract

DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK-psbI spacer, and trnH-psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.

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PMID:
19666622
PMCID:
PMC2722355
DOI:
10.1073/pnas.0905845106
[Indexed for MEDLINE]
Free PMC Article
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