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Biochim Biophys Acta. 2010 May;1804(5):1032-40. doi: 10.1016/j.bbapap.2009.07.021. Epub 2009 Aug 7.

Techniques used to study the DNA polymerase reaction pathway.

Author information

1
Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, P.O. Box 208114, New Haven, CT 06520-8114, USA. catherine.joyce@yale.edu

Abstract

A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.

PMID:
19665596
PMCID:
PMC2846202
DOI:
10.1016/j.bbapap.2009.07.021
[Indexed for MEDLINE]
Free PMC Article

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