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Anal Biochem. 2009 Dec 15;395(2):244-8. doi: 10.1016/j.ab.2009.08.002. Epub 2009 Aug 7.

Microsphere-based co-immunoprecipitation in multiplex.

Author information

1
NMI Natural and Medical Sciences Institute, University of Tübingen, 72770 Reutlingen, Germany. poetz@nmi.de

Abstract

Co-immunoprecipitation (co-IP) is a prominent technique for evaluating protein-protein interactions. Currently, large quantities of protein are required to perform co-IP followed by mass spectrometric or Western blot analyses of the interacting proteins. Here catenin-cadherin complexes were employed to establish a multiplexed microsphere-based co-immunoprecipitation (micro co-IP) protocol that allows the analysis of different complexes of a given protein with various interacting proteins within a single experiment using a limited amount of sample material.

PMID:
19665442
DOI:
10.1016/j.ab.2009.08.002
[Indexed for MEDLINE]

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