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Opt Express. 2009 Aug 3;17(16):13354-64.

Deep tissue multiphoton microscopy using longer wavelength excitation.

Author information

1
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA. dk287@cornell.edu

Abstract

We compare the maximal two-photon fluorescence microscopy (TPM) imaging depth achieved with 775-nm excitation to that achieved with 1280-nm excitation through in vivo and ex vivo TPM of fluorescently-labeled blood vessels in mouse brain. We achieved high contrast imaging of blood vessels at approximately twice the depth with 1280-nm excitation as with 775-nm excitation. An imaging depth of 1 mm can be achieved in in vivo imaging of adult mouse brains at 1280 nm with approximately 1-nJ pulse energy at the sample surface. Blood flow speed measurements at a depth of 900 mum are performed.

PMID:
19654740
[Indexed for MEDLINE]

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