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J Infect Dis. 2009 Sep 15;200(6):916-25. doi: 10.1086/605443.

Induction of microRNA-155 during Helicobacter pylori infection and its negative regulatory role in the inflammatory response.

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1
Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Southwest Hospital, Third Military Medical University, No. 30 Gaotanyan Street, Chongqing, China.

Abstract

BACKGROUND:

MicroRNAs (miRNAs) are small, noncoding RNAs that regulate gene expression at posttranscriptional level. H. pylori is a major human pathogenic bacterium in gastric mucosa. To date, the role of miRNAs in response to H. pylori infection has not been explored.

METHODS:

The expression profile of cellular miRNAs during H. pylori infection was analyzed by using microarray and quantitative reverse-transcriptase polymerase chain reaction. The potential target of miR-155 was identified by luciferase assay and Western blot. Promoter analysis and inhibitor experiment were used to investigate the pathway involved in the induction of miR-155. Examination of miR-155 function was performed by overexpression and inhibition of miR-155.

RESULTS:

H. pylori was able to increase the miR-155 expression in gastric epithelial cell lines and gastric mucosal tissues, and nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) pathway were required for the induction of miR-155. miR-155 may down-regulate IkappaB kinase epsilon, Sma- and Mad-related protein 2 (SMAD2), and Fas-associated death domain protein. Furthermore, the overexpression of miR-155 negatively regulated the release of interleukin-8 and growth-related oncogene-alpha.

CONCLUSIONS:

This study provides the first description of increased expression of miR-155 in H. pylori infection, and miR-155 may function as novel negative regulator that help to fine-tune the inflammation response of H. pylori infection.

PMID:
19650740
DOI:
10.1086/605443
[Indexed for MEDLINE]
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