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Clin Biochem. 2010 Jan;43(1-2):150-8. doi: 10.1016/j.clinbiochem.2009.07.020. Epub 2009 Jul 29.

Differential expression profiling of microRNAs and their potential involvement in renal cell carcinoma pathogenesis.

Author information

1
Department of Laboratory Medicine, and the Keenan Research Centre in the Li Ka Shing Knowledge Institute St Michael's Hospital, Toronto, Canada.

Abstract

OBJECTIVE:

We seek to identify the differentially expressed miRNAs in the clear cell subtype (ccRCC) of kidney cancer.

DESIGN AND METHODS:

We performed a miRNA microarray analysis to compare the miRNA expression levels between ccRCC tissues and their normal counterpart. The top dysregulated miRNAs were validated by quantitative RT-PCR analysis. Bioinformatics analysis was also performed.

RESULTS:

A total of 33 dysregulated miRNAs were identified in ccRCC, including 21 upregulated miRNAs and many of these miRNAs have been reported to be dysregulated in other malignancies and have a potential role in cancer pathogenesis. The miRNAs showed a significant correlation with reported chromosomal aberration sites. We also utilized target prediction algorithms to identify gene targets. Preliminary analyses showed these targets can be directly involved in RCC pathogenesis.

CONCLUSION:

We identified miRNAs that are dysregulated in ccRCC and bioinformatics analysis suggests that these miRNAs may be involved in cancer pathogenesis and have the potential to be biomarkers.

[Indexed for MEDLINE]

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