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J Gen Microbiol. 1990 Dec;136(12):2351-7.

A combined physical and genetic map of Pseudomonas aeruginosa PAO.

Author information

1
Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.

Abstract

A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.

PMID:
1964171
DOI:
10.1099/00221287-136-12-2351
[Indexed for MEDLINE]

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