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Biomol NMR Assign. 2009 Jun;3(1):77-80. doi: 10.1007/s12104-009-9145-9. Epub 2009 Jan 13.

Sequence-specific 1H N, 13C, and 15N backbone resonance assignments of the 34 kDa Paramecium bursaria Chlorella virus 1 (PBCV1) DNA ligase.

Author information

1
Department of Chemistry, The City College of New York, 160 Convent Avenue, New York, NY 10031, USA.

Abstract

Chlorella virus DNA ligase (ChVLig) is a minimal (298-amino acid) pluripotent ATP-dependent ligase composed of three structural modules--a nucleotidyltransferase domain, an OB domain, and a beta-hairpin latch--that forms a circumferential clamp around nicked DNA. ChVLig provides an instructive model to understand the chemical and conformational steps of nick repair. Here we report the assignment of backbone (13)C, (15)N, (1)H(N) resonances of this 34.2 kDa protein, the first for a DNA ligase in full-length form.

PMID:
19636951
PMCID:
PMC2746884
DOI:
10.1007/s12104-009-9145-9
[Indexed for MEDLINE]
Free PMC Article

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