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ACS Nano. 2009 Aug 25;3(8):2163-70. doi: 10.1021/nn900187k.

Adjustable ellipsoid nanoparticles assembled from re-engineered connectors of the bacteriophage phi29 DNA packaging motor.

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1
Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, Ohio 45221, USA.

Abstract

A 24 x 30 nm ellipsoid nanoparticle containing 84 subunits or 7 dodecamers of the re-engineered core protein of the bacteriophage phi29 DNA packaging motor was constructed. Homogeneous nanoparticles were obtained with simple one-step purification. Electron microscopy and analytical ultracentrifugation were employed to elucidate the structure, shape, size, and mechanism of assembly. The formation of this structure was mediated and stabilized by N-terminal peptide extensions. Reversal of the 84-subunit ellipsoid nanoparticle to its dodecamer subunit was controlled by the cleavage of the extended N-terminal peptide with a protease. The 84 outward-oriented C-termini were conjugated with a streptavidin binding peptide which can be used for the incorporation of markers. This further extends the application of this nanoparticle to pathogen detection and disease diagnosis by signal enhancement.

PMID:
19634910
PMCID:
PMC2731514
DOI:
10.1021/nn900187k
[Indexed for MEDLINE]
Free PMC Article
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