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Nat Methods. 2009 Aug;6(8):600-2. doi: 10.1038/nmeth.1349. Epub 2009 Jul 26.

The twin spot generator for differential Drosophila lineage analysis.

Author information

1
Commissariat à l'Energie Atomique, Institut de Recherches en Technologies et Sciences pour le Vivant, Centre National de la Recherche Scientifique, Grenoble, France. rgriffin@receptor.med.harvard.edu

Abstract

In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.

PMID:
19633664
PMCID:
PMC2720837
DOI:
10.1038/nmeth.1349
[Indexed for MEDLINE]
Free PMC Article

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