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Nat Methods. 2009 Aug;6(8):603-5. doi: 10.1038/nmeth.1356. Epub 2009 Jul 26.

G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila.

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1
Department of Molecular, Cell and Developmental Biology, Los Angeles, University of California, Los Angeles, Los Angeles, California, USA.

Abstract

We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.

PMID:
19633663
PMCID:
PMC2754220
DOI:
10.1038/nmeth.1356
[Indexed for MEDLINE]
Free PMC Article
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