a) FACS plots of CD4+Foxp3+ T cells (%) in spleen and VAT, 3 wk after transfer of purified CD4+ or purified CD4+Foxp3− (EGFPneg, 99.1% pure) cells, one of two similar plots shown. b) Change of HFD-RAGnull body weight 3 wk post transfer of CD4+, CD4+Foxp3−, and CD4+IL-10null T cells (n=5/group, *p<0.01, t-test). c) GTT (n=5/group, *p<0.0001, 2-way ANOVA). d) fasting glucose (left panel, n=5 mice/group, *p<0.01, t-test) and fasting insulin (right panel, n=5 mice/group, *p<0.01, t-test), 3 wk post transfer of CD4+, CD4+Foxp3−, or CD4+IL-10null T cells into HFD-RAGnull recipients. e) IL-4 (left panel) and IL-13 (right panel) produced by anti-CD3 plus anti-CD28 stimulated VAT T cells of 16 wk old HFD WT, and HFD RAGnull recipients 3 wk post transfer of CD4+ T cells (n=3/group). f) FACS plots for CD4+ gated T cells from VAT of HFD RAGnull mice that received either CD4+STAT6null (left panel) or CD4+WT (right panel) T cells 3 wk previously. g) Glucose tolerance 3 wk following transfer of CD4+ or CD4+STAT6null T cells (n=5/group, *p<0.05, 2-way ANOVA). h) Fasting glucose (left panel), fasting insulin (middle panel), and weight change (right panel) in HFD RAGnull recipients 3 wk post transfer of purified CD4+ or CD4+STAT6null T cells (n=5 mice/group, *p<0.01, t-test). i, Left panel: representative FACS plot CD4+GATA-3+ (%Th2) cells in spleen and VAT of 14–16 wk old regular diet and HFD B6 mice. j, Left panel: pooled data from (i, n=4/group, *p<0.03, t-test), right panel: total number of CD4+GATA-3+ (Th2) T cells/g of VAT in regular diet and HFD B6 mice (n=4/group, p>0.4, t-test).