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Carbohydr Res. 2009 Sep 8;344(13):1687-9. doi: 10.1016/j.carres.2009.06.018. Epub 2009 Jun 21.

Isolation and purification of D-mannose from palm kernel.

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Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.


An economically viable procedure for the isolation and purification of d-mannose from palm kernel was developed in this research. The palm kernel was catalytically hydrolyzed with sulfuric acid at 100 degrees C and then fermented by mannan-degrading enzymes. The solution after fermentation underwent filtration in a silica gel column, desalination by ion-exchange resin, and crystallization in ethanol to produce pure d-mannose in a total yield of 48.4% (based on the weight of the palm kernel). Different enzymes were investigated, and the results indicated that endo-beta-mannanase was the best enzyme to promote the hydrolysis of the oligosaccharides isolated from the palm kernel. The pure d-mannose sample was characterized by FTIR, (1)H NMR, and (13)C NMR spectra.

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