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J Pharm Biomed Anal. 2009 Dec 5;50(5):954-8. doi: 10.1016/j.jpba.2009.06.038. Epub 2009 Jun 27.

A high-throughput test to detect C.E.R.A. doping in blood.

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  • 1Laboratoire Suisse d'Analyse du Dopage, Centre Universitaire Romand de Médecine Légale, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Croisettes 22, 1066 Epalinges, Switzerland.

Abstract

C.E.R.A., a continuous erythropoietin receptor activator, is a new third-generation erythropoiesis-stimulating agent (ESA) that has recently been linked with abuse in endurance sports. In order to combat this new form of doping, we examined an enzyme-linked immunosorbent assay (ELISA) designed to detect the presence of C.E.R.A. in serum samples. The performance of the assay was evaluated using a pilot excretion study that involved six subjects receiving C.E.R.A. Validation data demonstrated an excellent reproducibility and ensured the applicability of the assay for anti-doping purposes. To maximize the chances of detecting the drug in serum samples, we propose the use of this specific ELISA test as a high-throughput screening method, combined with a classic isoelectric focusing test as a confirmatory assay. This strategy should make C.E.R.A. abuse relatively easy to detect, thereby preventing the future use of this drug as a doping agent.

PMID:
19625154
DOI:
10.1016/j.jpba.2009.06.038
[PubMed - indexed for MEDLINE]
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