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J Biol Chem. 2009 Sep 25;284(39):26243-50. doi: 10.1074/jbc.M109.031179. Epub 2009 Jul 21.

Leucyl-tRNA synthetase-dependent and -independent activation of a group I intron.

Author information

1
Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA.

Abstract

Leucyl-tRNA synthetase (LeuRS) is an essential RNA splicing factor for yeast mitochondrial introns. Intracellular experiments have suggested that it works in collaboration with a maturase that is encoded within the bI4 intron. RNA deletion mutants of the large bI4 intron were constructed to identify a competently folded intron for biochemical analysis. The minimized bI4 intron was active in RNA splicing and contrasts with previous proposals that the canonical core of the bI4 intron is deficient for catalysis. The activity of the minimized bI4 intron was enhanced in vitro by the presence of the bI4 maturase or LeuRS.

PMID:
19622748
PMCID:
PMC2785312
DOI:
10.1074/jbc.M109.031179
[Indexed for MEDLINE]
Free PMC Article

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