Format

Send to

Choose Destination
Cytogenet Genome Res. 2009;125(1):74-80. doi: 10.1159/000218749. Epub 2009 Jul 14.

Cytogenetic analysis of Populus trichocarpa--ribosomal DNA, telomere repeat sequence, and marker-selected BACs.

Author information

1
Southern Institute of Forest Genetics, Southern Research Station, U.S. Forest Service, Forest Tree Molecular Cytogenetics Laboratory, Department of Ecosystem Science and Management, Texas A&M University, College Station, TX, USA. nfaridi@tamu.edu

Abstract

The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.

PMID:
19617699
DOI:
10.1159/000218749
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for S. Karger AG, Basel, Switzerland
Loading ...
Support Center