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J Immunol Methods. 1991 Nov 22;144(2):247-51.

A simple method for the purification of human peripheral blood monocytes. A substitute for Sepracell-MN.

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Division of Hematology, Albany Medical College, NY 12208-3479.


Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient which matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 10(6) monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN.

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