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Anal Chem. 2009 Aug 15;81(16):7102-6. doi: 10.1021/ac901234r.

Gaussia luciferase variant for high-throughput functional screening applications.

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  • 1Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston Massachusetts 02115, USA.

Abstract

Gaussia luciferase (Gluc) is a sensitive reporter for studying different biological processes such as gene expression, promoter activity, protein-protein interactions, signal transduction, as well as tumor cell growth and response to therapy. Since Gluc is naturally secreted, the kinetics of these processes can be monitored in real-time by measuring an aliquot of conditioned medium in culture or a few microliters of blood in vivo at different time points. Gluc catalyzes light emission with a short half-life which is unfavorable for certain applications. We isolated a Gluc mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications.

PMID:
19601604
PMCID:
PMC2846205
DOI:
10.1021/ac901234r
[PubMed - indexed for MEDLINE]
Free PMC Article
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